(2017) Bioresource Technology_Estimating total lipid content of Camelina sativa via pyrolysis assisted in-situ transesterification with dimethyl carbonate

Jung J.-M., Lee J., Oh J.-I., Kim H.-W., Kwon E.E.

(Elsevier Ltd) Bioresource Technology ISSN: 9608524 Vol.225 Issue. Article No. DOI: 10.1016/j.biortech.2016.11.061

Direct derivatization of C. sativa seed into FAMEs without lipid extraction was conducted for the quantification of lipid analysis via in-situ thermal methylation with dimethyl carbonate as an acyl acceptor on silica (SiO₂). The introduced method had an extraordinarily high tolerance against impurities such as pyrolytic products and moisture. To ensure the technical completeness of in-situ methylation, thermal cracking of FAMEs transformed from C. sativa seed was also explored. Thermal cracking of unsaturated FAMEs such as C18:1, C18:2, C18:3 and C20:1 occurred at temperatures higher than 365 °C due to their thermal instability. Thus, experimental findings in this study suggests not only that qualitative analysis of fatty acid profile in C. sativa seed via in-situ methylation using SiO₂ could be achieve, but also that the total lipid content (42.65 wt.%) in C. sativa seed could be accurately estimated. © 2016 Elsevier Ltd

This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government ( MSIP ) (No. 2014RA1A004893 ). This work was also supported by a National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (No. NRF-2015H1D3A1066513 ).